This thoroughly revised and updated edition of a widely used practical guide toflow cytometry describes in stepbystep detail an array of time proven andcuttinge... » 

This thoroughly revised and updated edition of a widely used practical guide toflow cytometry describes in stepbystep detail an array of time proven andcuttingedge techniques much needed in todays advanced laboratories. Thesereadily reproducible methods deploy emerging flow cytometry technologies inmany new applications especially in the field of stem cells functionalgenomics and proteomics and microbiology. Here the aspiring investigator willfind methods for the characterization of stemprogenitor cells by monitoringthe efflux of fluorescent dyes and the elucidation of signal transductionpathways using phosphospecific antibodies. There are also techniques formonitoring gene transfer and expression using fluorescent protein technologyhigh throughput screening for discovery of novel protein interactionsphenotypic and functional characterization of T cell subsets and precursorsand microbial flow cytometry to highlight but some of the many usefulprocedures. «